Construct: shRNA TRCN0000023189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007713.2-613s1c1
DNA Barcode:
GCCCTGAAGATCATCCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clk3 (102414)
Gene Description:
CDC-like kinase 3
Transcript:
RefSeq NM_007713.2 (NON-CURRENT)
Match location:
Position 613 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGAAGATCATCCGTAAT
Hairpin Sequence:
5'-CCGG-GCCCTGAAGATCATCCGTAAT-CTCGAG-ATTACGGATGATCTTCAGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGAAGATCATCCGTAATCTCGAGATTACGGATGATCTTCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGAAGATCATCCGTAATCTCGAGATTACGGATGATCTTCAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000231374