Construct: shRNA TRCN0000023202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015806.2-1490s1c1
DNA Barcode:
CGAGAGAAGTATCTAGAGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapk6 (50772)
Gene Description:
mitogen-activated protein kinase 6
Transcript:
RefSeq NM_015806.2 (NON-CURRENT)
Match location:
Position 1490 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAGAAGTATCTAGAGGAT
Hairpin Sequence:
5'-CCGG-CGAGAGAAGTATCTAGAGGAT-CTCGAG-ATCCTCTAGATACTTCTCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAGAAGTATCTAGAGGATCTCGAGATCCTCTAGATACTTCTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAGAAGTATCTAGAGGATCTCGAGATCCTCTAGATACTTCTCTCG-3'

Other clones with same target sequence:

  1. TRCN0000345192