Construct: shRNA TRCN0000023229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130860.1-421s1c1
DNA Barcode:
CGTCTTAGTCAAGTTCACGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk9 (107951)
Gene Description:
cyclin-dependent kinase 9 (CDC2-related kinase)
Transcript:
RefSeq NM_130860.1 (NON-CURRENT)
Match location:
Position 421 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTTAGTCAAGTTCACGTT
Hairpin Sequence:
5'-CCGG-CGTCTTAGTCAAGTTCACGTT-CTCGAG-AACGTGAACTTGACTAAGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTTAGTCAAGTTCACGTTCTCGAGAACGTGAACTTGACTAAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTTAGTCAAGTTCACGTTCTCGAGAACGTGAACTTGACTAAGACG-3'

Other clones with same target sequence:

(none)