Construct: shRNA TRCN0000023271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_142163.2-79s1c1
DNA Barcode:
CTGGATTCATTCGTGAAGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC245619 (245619)
Gene Description:
glycogen synthase kinase 3 alpha probable pseudogene
Transcript:
RefSeq XM_142163.2 (NON-CURRENT)
Match location:
Position 79 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGATTCATTCGTGAAGGAT
Hairpin Sequence:
5'-CCGG-CTGGATTCATTCGTGAAGGAT-CTCGAG-ATCCTTCACGAATGAATCCAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGATTCATTCGTGAAGGATCTCGAGATCCTTCACGAATGAATCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGATTCATTCGTGAAGGATCTCGAGATCCTTCACGAATGAATCCAG-3'

Other clones with same target sequence:

(none)