Construct: shRNA TRCN0000023310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009465.2-2332s1c1
DNA Barcode:
CGTCAAGGAAATCGGCTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Axl (26362)
Gene Description:
AXL receptor tyrosine kinase
Transcript:
RefSeq NM_009465.2 (NON-CURRENT)
Match location:
Position 2332 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAAGGAAATCGGCTGAAA
Hairpin Sequence:
5'-CCGG-CGTCAAGGAAATCGGCTGAAA-CTCGAG-TTTCAGCCGATTTCCTTGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAAGGAAATCGGCTGAAACTCGAGTTTCAGCCGATTTCCTTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAAGGAAATCGGCTGAAACTCGAGTTTCAGCCGATTTCCTTGACG-3'

Other clones with same target sequence:

  1. TRCN0000322132