Construct: shRNA TRCN0000023366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_145594.4-196s1c1
DNA Barcode:
GATGTTCGTTTCAAGGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC243968 (243968)
Gene Description:
similar to KIAA1883 protein
Transcript:
RefSeq XM_145594.4 (NON-CURRENT)
Match location:
Position 196 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTTCGTTTCAAGGAGTTT
Hairpin Sequence:
5'-CCGG-GATGTTCGTTTCAAGGAGTTT-CTCGAG-AAACTCCTTGAAACGAACATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTTCGTTTCAAGGAGTTTCTCGAGAAACTCCTTGAAACGAACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTTCGTTTCAAGGAGTTTCTCGAGAAACTCCTTGAAACGAACATC-3'

Other clones with same target sequence:

(none)