Construct: shRNA TRCN0000023383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008054.1-364s1c1
DNA Barcode:
CATCCCGAACTACAACAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fyn (14360)
Gene Description:
Fyn proto-oncogene
Transcript:
RefSeq NM_008054.1 (NON-CURRENT)
Match location:
Position 364 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCCGAACTACAACAACTT
Hairpin Sequence:
5'-CCGG-CATCCCGAACTACAACAACTT-CTCGAG-AAGTTGTTGTAGTTCGGGATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCCGAACTACAACAACTTCTCGAGAAGTTGTTGTAGTTCGGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCCGAACTACAACAACTTCTCGAGAAGTTGTTGTAGTTCGGGATG-3'

Other clones with same target sequence:

(none)