Construct: shRNA TRCN0000023388

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_109715.3-689s1c1
DNA Barcode:
CCTCCGTAAGAATAACCAGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Erbb2 (13866)
Gene Description:
erb-b2 receptor tyrosine kinase 2
Transcript:
RefSeq XM_109715.3 (NON-CURRENT)
Match location:
Position 689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023388)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCGTAAGAATAACCAGCT
Hairpin Sequence:
5'-CCGG-CCTCCGTAAGAATAACCAGCT-CTCGAG-AGCTGGTTATTCTTACGGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCGTAAGAATAACCAGCTCTCGAGAGCTGGTTATTCTTACGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCGTAAGAATAACCAGCTCTCGAGAGCTGGTTATTCTTACGGAGG-3'

Other clones with same target sequence:

(none)