Construct: shRNA TRCN0000023399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010140.1-1611s1c1
DNA Barcode:
GCCAGATACTACTTATGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epha3 (13837)
Gene Description:
Eph receptor A3
Transcript:
RefSeq NM_010140.1 (NON-CURRENT)
Match location:
Position 1611 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGATACTACTTATGTCTT
Hairpin Sequence:
5'-CCGG-GCCAGATACTACTTATGTCTT-CTCGAG-AAGACATAAGTAGTATCTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGATACTACTTATGTCTTCTCGAGAAGACATAAGTAGTATCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGATACTACTTATGTCTTCTCGAGAAGACATAAGTAGTATCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000174051