Construct: shRNA TRCN0000023410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007549.2-1783s1c1
DNA Barcode:
CCACCGGAGTTGTACAATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Blk (12143)
Gene Description:
B lymphoid kinase
Transcript:
RefSeq NM_007549.2 (CURRENT)
Match location:
Position 1783 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCGGAGTTGTACAATGAT
Hairpin Sequence:
5'-CCGG-CCACCGGAGTTGTACAATGAT-CTCGAG-ATCATTGTACAACTCCGGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCGGAGTTGTACAATGATCTCGAGATCATTGTACAACTCCGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCGGAGTTGTACAATGATCTCGAGATCATTGTACAACTCCGGTGG-3'

Other clones with same target sequence:

(none)