Construct: shRNA TRCN0000023417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356664.1-154s1c1
DNA Barcode:
GAGGCTGATGTCCTCATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC382761 (382761)
Gene Description:
similar to BDNF/NT-3 growth factors receptor precursor (TrkB tyrosine kinase) (GP145-TrkB/GP95-TrkB) (Trk-B)
Transcript:
RefSeq XM_356664.1 (NON-CURRENT)
Match location:
Position 154 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCTGATGTCCTCATTAAA
Hairpin Sequence:
5'-CCGG-GAGGCTGATGTCCTCATTAAA-CTCGAG-TTTAATGAGGACATCAGCCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCTGATGTCCTCATTAAACTCGAGTTTAATGAGGACATCAGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCTGATGTCCTCATTAAACTCGAGTTTAATGAGGACATCAGCCTC-3'

Other clones with same target sequence:

(none)