Construct: shRNA TRCN0000023419

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355556.1-557s1c1
DNA Barcode:
GCCCACCTGAAGTCTTTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm1893 (381599)
Gene Description:
gene model 1893, (NCBI)
Transcript:
RefSeq XM_355556.1 (NON-CURRENT)
Match location:
Position 557 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023419)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCACCTGAAGTCTTTCATT
Hairpin Sequence:
5'-CCGG-GCCCACCTGAAGTCTTTCATT-CTCGAG-AATGAAAGACTTCAGGTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCACCTGAAGTCTTTCATTCTCGAGAATGAAAGACTTCAGGTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCACCTGAAGTCTTTCATTCTCGAGAATGAAAGACTTCAGGTGGGC-3'

Other clones with same target sequence:

(none)