Construct: shRNA TRCN0000023469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010208.2-853s1c1
DNA Barcode:
CGGCACTACATGGAAGTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fgr (14191)
Gene Description:
FGR proto-oncogene, Src family tyrosine kinase
Transcript:
RefSeq NM_010208.2 (NON-CURRENT)
Match location:
Position 853 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCACTACATGGAAGTGAAT
Hairpin Sequence:
5'-CCGG-CGGCACTACATGGAAGTGAAT-CTCGAG-ATTCACTTCCATGTAGTGCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCACTACATGGAAGTGAATCTCGAGATTCACTTCCATGTAGTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCACTACATGGAAGTGAATCTCGAGATTCACTTCCATGTAGTGCCG-3'

Other clones with same target sequence:

(none)