Construct: shRNA TRCN0000023485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007982.1-1523s1c1
DNA Barcode:
GCCTTAACAATGCGTCAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptk2 (14083)
Gene Description:
PTK2 protein tyrosine kinase 2
Transcript:
RefSeq NM_007982.1 (NON-CURRENT)
Match location:
Position 1523 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTAACAATGCGTCAGTTT
Hairpin Sequence:
5'-CCGG-GCCTTAACAATGCGTCAGTTT-CTCGAG-AAACTGACGCATTGTTAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTAACAATGCGTCAGTTTCTCGAGAAACTGACGCATTGTTAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTAACAATGCGTCAGTTTCTCGAGAAACTGACGCATTGTTAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000121130
  2. TRCN0000350696