Construct: shRNA TRCN0000023546

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009074.1-440s1c1
DNA Barcode:
GCCTACGAGGACAGTACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mst1r (19882)
Gene Description:
macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Transcript:
RefSeq NM_009074.1 (NON-CURRENT)
Match location:
Position 440 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023546)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACGAGGACAGTACAAAT
Hairpin Sequence:
5'-CCGG-GCCTACGAGGACAGTACAAAT-CTCGAG-ATTTGTACTGTCCTCGTAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACGAGGACAGTACAAATCTCGAGATTTGTACTGTCCTCGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACGAGGACAGTACAAATCTCGAGATTTGTACTGTCCTCGTAGGC-3'

Other clones with same target sequence:

(none)