Construct: shRNA TRCN0000023549

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022563.1-1638s1c1
DNA Barcode:
CCACCTATGATCCCATGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddr2 (18214)
Gene Description:
discoidin domain receptor family, member 2
Transcript:
RefSeq NM_022563.1 (NON-CURRENT)
Match location:
Position 1638 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023549)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTATGATCCCATGCTTA
Hairpin Sequence:
5'-CCGG-CCACCTATGATCCCATGCTTA-CTCGAG-TAAGCATGGGATCATAGGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTATGATCCCATGCTTACTCGAGTAAGCATGGGATCATAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTATGATCCCATGCTTACTCGAGTAAGCATGGGATCATAGGTGG-3'

Other clones with same target sequence:

(none)