Construct: shRNA TRCN0000023557
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013690.1-538s1c1
- DNA Barcode:
- GCCTTAATGAACCAGCACCAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Tek (21687)
- Gene Description:
- endothelial-specific receptor tyrosine kinase
- Transcript:
- RefSeq NM_013690.1 (NON-CURRENT)
- Match location:
- Position 538 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000023557)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTTAATGAACCAGCACCAA
- Hairpin Sequence:
-
5'-CCGG-GCCTTAATGAACCAGCACCAA-CTCGAG-TTGGTGCTGGTTCATTAAGGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTTAATGAACCAGCACCAACTCGAGTTGGTGCTGGTTCATTAAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTTAATGAACCAGCACCAACTCGAGTTGGTGCTGGTTCATTAAGGC-3'
Other clones with same target sequence:
(none)
