Construct: shRNA TRCN0000023571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011518.1-675s1c1
DNA Barcode:
GAGGGAACTTAATGGCACCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Syk (20963)
Gene Description:
spleen tyrosine kinase
Transcript:
RefSeq NM_011518.1 (NON-CURRENT)
Match location:
Position 675 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGGAACTTAATGGCACCTA
Hairpin Sequence:
5'-CCGG-GAGGGAACTTAATGGCACCTA-CTCGAG-TAGGTGCCATTAAGTTCCCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGGAACTTAATGGCACCTACTCGAGTAGGTGCCATTAAGTTCCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGGAACTTAATGGCACCTACTCGAGTAGGTGCCATTAAGTTCCCTC-3'

Other clones with same target sequence:

(none)