Construct: shRNA TRCN0000023591

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008587.1-410s1c1
DNA Barcode:
GCTGGCATTTCATGGTGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mertk (17289)
Gene Description:
c-mer proto-oncogene tyrosine kinase
Transcript:
RefSeq NM_008587.1 (NON-CURRENT)
Match location:
Position 410 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023591)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGCATTTCATGGTGGAAA
Hairpin Sequence:
5'-CCGG-GCTGGCATTTCATGGTGGAAA-CTCGAG-TTTCCACCATGAAATGCCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGCATTTCATGGTGGAAACTCGAGTTTCCACCATGAAATGCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGCATTTCATGGTGGAAACTCGAGTTTCCACCATGAAATGCCAGC-3'

Other clones with same target sequence:

(none)