Construct: shRNA TRCN0000023613

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175168.2-2728s1c1
DNA Barcode:
CCTGGGAGATCTCAAACAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptk7 (71461)
Gene Description:
PTK7 protein tyrosine kinase 7
Transcript:
RefSeq NM_175168.2 (NON-CURRENT)
Match location:
Position 2728 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023613)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGAGATCTCAAACAGTT
Hairpin Sequence:
5'-CCGG-CCTGGGAGATCTCAAACAGTT-CTCGAG-AACTGTTTGAGATCTCCCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGAGATCTCAAACAGTTCTCGAGAACTGTTTGAGATCTCCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGAGATCTCAAACAGTTCTCGAGAACTGTTTGAGATCTCCCAGG-3'

Other clones with same target sequence:

(none)