Construct: shRNA TRCN0000023625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_194688.1-109s1c1
DNA Barcode:
CGAGCCTTGCGCAAATACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm642 (271709)
Gene Description:
gene model 642, (NCBI)
Transcript:
RefSeq XM_194688.1 (NON-CURRENT)
Match location:
Position 109 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCCTTGCGCAAATACTAT
Hairpin Sequence:
5'-CCGG-CGAGCCTTGCGCAAATACTAT-CTCGAG-ATAGTATTTGCGCAAGGCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCCTTGCGCAAATACTATCTCGAGATAGTATTTGCGCAAGGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCCTTGCGCAAATACTATCTCGAGATAGTATTTGCGCAAGGCTCG-3'

Other clones with same target sequence:

(none)