Construct: shRNA TRCN0000023631

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172498.1-2242s1c1
DNA Barcode:
CCTCAGTGACATTTATCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptk2b (19229)
Gene Description:
PTK2 protein tyrosine kinase 2 beta
Transcript:
RefSeq NM_172498.1 (NON-CURRENT)
Match location:
Position 2242 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023631)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGTGACATTTATCAGAT
Hairpin Sequence:
5'-CCGG-CCTCAGTGACATTTATCAGAT-CTCGAG-ATCTGATAAATGTCACTGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGTGACATTTATCAGATCTCGAGATCTGATAAATGTCACTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGTGACATTTATCAGATCTCGAGATCTGATAAATGTCACTGAGG-3'

Other clones with same target sequence:

(none)