Construct: shRNA TRCN0000023634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356004.1-53s1c1
DNA Barcode:
GTACAAGAGAAAGAGAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381968 (381968)
Gene Description:
similar to keratinocyte growth factor receptor precursor
Transcript:
RefSeq XM_356004.1 (NON-CURRENT)
Match location:
Position 53 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACAAGAGAAAGAGAAGAAA
Hairpin Sequence:
5'-CCGG-GTACAAGAGAAAGAGAAGAAA-CTCGAG-TTTCTTCTCTTTCTCTTGTAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACAAGAGAAAGAGAAGAAACTCGAGTTTCTTCTCTTTCTCTTGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACAAGAGAAAGAGAAGAAACTCGAGTTTCTTCTCTTTCTCTTGTAC-3'

Other clones with same target sequence:

(none)