Construct: shRNA TRCN0000023642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008000.1-1858s1c1
DNA Barcode:
GCTCACTGTCATCAAGAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fer (14158)
Gene Description:
fer (fms/fps related) protein kinase
Transcript:
RefSeq NM_008000.1 (NON-CURRENT)
Match location:
Position 1858 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACTGTCATCAAGAAGAT
Hairpin Sequence:
5'-CCGG-GCTCACTGTCATCAAGAAGAT-CTCGAG-ATCTTCTTGATGACAGTGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACTGTCATCAAGAAGATCTCGAGATCTTCTTGATGACAGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACTGTCATCAAGAAGATCTCGAGATCTTCTTGATGACAGTGAGC-3'

Other clones with same target sequence:

(none)