Construct: shRNA TRCN0000023646

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007680.2-2795s1c1
DNA Barcode:
CCCGAAGTGTGCTAGTGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ephb6 (13848)
Gene Description:
Eph receptor B6
Transcript:
RefSeq NM_007680.2 (NON-CURRENT)
Match location:
Position 2795 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023646)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAAGTGTGCTAGTGAATA
Hairpin Sequence:
5'-CCGG-CCCGAAGTGTGCTAGTGAATA-CTCGAG-TATTCACTAGCACACTTCGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAAGTGTGCTAGTGAATACTCGAGTATTCACTAGCACACTTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAAGTGTGCTAGTGAATACTCGAGTATTCACTAGCACACTTCGGG-3'

Other clones with same target sequence:

  1. TRCN0000321894