Construct: shRNA TRCN0000023714

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010207.1-1637s1c1
DNA Barcode:
CGGGTAATTCTATCGGGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fgfr2 (14183)
Gene Description:
fibroblast growth factor receptor 2
Transcript:
RefSeq NM_010207.1 (NON-CURRENT)
Match location:
Position 1637 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023714)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGTAATTCTATCGGGATAT
Hairpin Sequence:
5'-CCGG-CGGGTAATTCTATCGGGATAT-CTCGAG-ATATCCCGATAGAATTACCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGTAATTCTATCGGGATATCTCGAGATATCCCGATAGAATTACCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGTAATTCTATCGGGATATCTCGAGATATCCCGATAGAATTACCCG-3'

Other clones with same target sequence:

  1. TRCN0000321969