Construct: shRNA TRCN0000023758
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008029.1-1667s1c1
- DNA Barcode:
- CCAGGATGAGAGACTCATCTA
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Flt4 (14257)
- Gene Description:
- FMS-like tyrosine kinase 4
- Transcript:
- RefSeq NM_008029.1 (NON-CURRENT)
- Match location:
- Position 1667 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CCAGGATGAGAGACTCATCTA
- Hairpin Sequence:
- 5'-CCGG-CCAGGATGAGAGACTCATCTA-CTCGAG-TAGATGAGTCTCTCATCCTGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGGATGAGAGACTCATCTACTCGAGTAGATGAGTCTCTCATCCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGGATGAGAGACTCATCTACTCGAGTAGATGAGTCTCTCATCCTGG-3'