Construct: shRNA TRCN0000023759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027260.1-1094s1c1
DNA Barcode:
CCAGCTAAGTTCCCAAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vrk2 (69922)
Gene Description:
vaccinia related kinase 2
Transcript:
RefSeq NM_027260.1 (NON-CURRENT)
Match location:
Position 1094 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTAAGTTCCCAAAGAAA
Hairpin Sequence:
5'-CCGG-CCAGCTAAGTTCCCAAAGAAA-CTCGAG-TTTCTTTGGGAACTTAGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTAAGTTCCCAAAGAAACTCGAGTTTCTTTGGGAACTTAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTAAGTTCCCAAAGAAACTCGAGTTTCTTTGGGAACTTAGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000276621