Construct: shRNA TRCN0000023765

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152809.1-424s1c1
DNA Barcode:
GCAGAGTAGAAGAGATGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csnk1g3 (70425)
Gene Description:
casein kinase 1, gamma 3
Transcript:
RefSeq NM_152809.1 (NON-CURRENT)
Match location:
Position 424 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023765)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGTAGAAGAGATGATTT
Hairpin Sequence:
5'-CCGG-GCAGAGTAGAAGAGATGATTT-CTCGAG-AAATCATCTCTTCTACTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGTAGAAGAGATGATTTCTCGAGAAATCATCTCTTCTACTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGTAGAAGAGATGATTTCTCGAGAAATCATCTCTTCTACTCTGC-3'

Other clones with same target sequence:

(none)