Construct: shRNA TRCN0000023810

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_140038.3-1363s1c1
DNA Barcode:
GCCATGTTTGGAGTGGTCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC210619 (210619)
Gene Description:
similar to RP3-330M21.4
Transcript:
RefSeq XM_140038.3 (NON-CURRENT)
Match location:
Position 1363 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023810)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTTTGGAGTGGTCAAT
Hairpin Sequence:
5'-CCGG-GCCATGTTTGGAGTGGTCAAT-CTCGAG-ATTGACCACTCCAAACATGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTTTGGAGTGGTCAATCTCGAGATTGACCACTCCAAACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTTTGGAGTGGTCAATCTCGAGATTGACCACTCCAAACATGGC-3'

Other clones with same target sequence:

(none)