Construct: shRNA TRCN0000023813
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_140038.3-1071s1c1
- DNA Barcode:
- CTGGAGGAAGATCAAAGACAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC210619 (210619)
- Gene Description:
- similar to RP3-330M21.4
- Transcript:
- RefSeq XM_140038.3 (NON-CURRENT)
- Match location:
- Position 1071 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000023813)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTGGAGGAAGATCAAAGACAA
- Hairpin Sequence:
-
5'-CCGG-CTGGAGGAAGATCAAAGACAA-CTCGAG-TTGTCTTTGATCTTCCTCCAG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTGGAGGAAGATCAAAGACAACTCGAGTTGTCTTTGATCTTCCTCCAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTGGAGGAAGATCAAAGACAACTCGAGTTGTCTTTGATCTTCCTCCAG-3'
Other clones with same target sequence:
(none)
