Construct: shRNA TRCN0000023818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177174.2-814s1c1
DNA Barcode:
TGGGTCTTCTTCATCTGGAGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C330049O21Rik (320493)
Gene Description:
RIKEN cDNA C330049O21 gene
Transcript:
RefSeq NM_177174.2 (NON-CURRENT)
Match location:
Position 814 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGTCTTCTTCATCTGGAGT
Hairpin Sequence:
5'-CCGG-TGGGTCTTCTTCATCTGGAGT-CTCGAG-ACTCCAGATGAAGAAGACCCA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGTCTTCTTCATCTGGAGTCTCGAGACTCCAGATGAAGAAGACCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGTCTTCTTCATCTGGAGTCTCGAGACTCCAGATGAAGAAGACCCA-3'

Other clones with same target sequence:

(none)