Construct: shRNA TRCN0000023819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007908.2-1207s1c1
DNA Barcode:
GCTCTCTTCTTCTACTCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eef2k (13631)
Gene Description:
eukaryotic elongation factor-2 kinase
Transcript:
RefSeq NM_007908.2 (NON-CURRENT)
Match location:
Position 1207 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCTTCTTCTACTCTCAT
Hairpin Sequence:
5'-CCGG-GCTCTCTTCTTCTACTCTCAT-CTCGAG-ATGAGAGTAGAAGAAGAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCTTCTTCTACTCTCATCTCGAGATGAGAGTAGAAGAAGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCTTCTTCTACTCTCATCTCGAGATGAGAGTAGAAGAAGAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000321802