Construct: shRNA TRCN0000023909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_147046.2-455s1c1
DNA Barcode:
GCTACTAGAATTATGCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atr (245000)
Gene Description:
ataxia telangiectasia and Rad3 related
Transcript:
RefSeq XM_147046.2 (NON-CURRENT)
Match location:
Position 455 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACTAGAATTATGCAACAA
Hairpin Sequence:
5'-CCGG-GCTACTAGAATTATGCAACAA-CTCGAG-TTGTTGCATAATTCTAGTAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACTAGAATTATGCAACAACTCGAGTTGTTGCATAATTCTAGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACTAGAATTATGCAACAACTCGAGTTGTTGCATAATTCTAGTAGC-3'

Other clones with same target sequence:

(none)