Construct: shRNA TRCN0000023932

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_125706.5-3052s1c1
DNA Barcode:
GTCATCGACATGAATGGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcr (110279)
Gene Description:
breakpoint cluster region
Transcript:
RefSeq XM_125706.5 (NON-CURRENT)
Match location:
Position 3052 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023932)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATCGACATGAATGGAATT
Hairpin Sequence:
5'-CCGG-GTCATCGACATGAATGGAATT-CTCGAG-AATTCCATTCATGTCGATGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATCGACATGAATGGAATTCTCGAGAATTCCATTCATGTCGATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATCGACATGAATGGAATTCTCGAGAATTCCATTCATGTCGATGAC-3'

Other clones with same target sequence:

(none)