Construct: shRNA TRCN0000023935

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355107.1-118s1c1
DNA Barcode:
CCCGAGAACAACATCCCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381181 (381181)
Gene Description:
similar to heart alpha-kinase
Transcript:
RefSeq XM_355107.1 (NON-CURRENT)
Match location:
Position 118 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023935)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAGAACAACATCCCATAT
Hairpin Sequence:
5'-CCGG-CCCGAGAACAACATCCCATAT-CTCGAG-ATATGGGATGTTGTTCTCGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAGAACAACATCCCATATCTCGAGATATGGGATGTTGTTCTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAGAACAACATCCCATATCTCGAGATATGGGATGTTGTTCTCGGG-3'

Other clones with same target sequence:

(none)