Construct: shRNA TRCN0000023950

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012025.3-1122s1c1
DNA Barcode:
CGTCTCAAAGACGGTTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Racgap1 (26934)
Gene Description:
Rac GTPase-activating protein 1
Transcript:
RefSeq NM_012025.3 (NON-CURRENT)
Match location:
Position 1122 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023950)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTCAAAGACGGTTATTAA
Hairpin Sequence:
5'-CCGG-CGTCTCAAAGACGGTTATTAA-CTCGAG-TTAATAACCGTCTTTGAGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTCAAAGACGGTTATTAACTCGAGTTAATAACCGTCTTTGAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTCAAAGACGGTTATTAACTCGAGTTAATAACCGTCTTTGAGACG-3'

Other clones with same target sequence:

  1. TRCN0000322156