Construct: shRNA TRCN0000023954

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021450.1-4488s1c1
DNA Barcode:
CCAAAGATCAAGAACCCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trpm7 (58800)
Gene Description:
transient receptor potential cation channel, subfamily M, member 7
Transcript:
RefSeq NM_021450.1 (NON-CURRENT)
Match location:
Position 4488 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023954)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGATCAAGAACCCATTT
Hairpin Sequence:
5'-CCGG-CCAAAGATCAAGAACCCATTT-CTCGAG-AAATGGGTTCTTGATCTTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGATCAAGAACCCATTTCTCGAGAAATGGGTTCTTGATCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGATCAAGAACCCATTTCTCGAGAAATGGGTTCTTGATCTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000274773