Construct: shRNA TRCN0000024148

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011492.1-543s1c1
DNA Barcode:
CGCCAAGCTCATCGGCAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stk11 (20869)
Gene Description:
serine/threonine kinase 11
Transcript:
RefSeq NM_011492.1 (NON-CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024148)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAAGCTCATCGGCAAGTA
Hairpin Sequence:
5'-CCGG-CGCCAAGCTCATCGGCAAGTA-CTCGAG-TACTTGCCGATGAGCTTGGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAAGCTCATCGGCAAGTACTCGAGTACTTGCCGATGAGCTTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAAGCTCATCGGCAAGTACTCGAGTACTTGCCGATGAGCTTGGCG-3'

Other clones with same target sequence:

  1. TRCN0000274658