Construct: shRNA TRCN0000024179

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178597.2-221s1c1
DNA Barcode:
GCCCGAGATCATCAGAAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Camk2g (12325)
Gene Description:
calcium/calmodulin-dependent protein kinase II gamma
Transcript:
RefSeq NM_178597.2 (NON-CURRENT)
Match location:
Position 221 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024179)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGAGATCATCAGAAACTA
Hairpin Sequence:
5'-CCGG-GCCCGAGATCATCAGAAACTA-CTCGAG-TAGTTTCTGATGATCTCGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGAGATCATCAGAAACTACTCGAGTAGTTTCTGATGATCTCGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGAGATCATCAGAAACTACTCGAGTAGTTTCTGATGATCTCGGGC-3'

Other clones with same target sequence:

(none)