Construct: shRNA TRCN0000024275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016695.1-1112s1c1
DNA Barcode:
CGCCATGAGCTGCTCATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mpp2 (50997)
Gene Description:
membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
Transcript:
RefSeq NM_016695.1 (NON-CURRENT)
Match location:
Position 1112 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCATGAGCTGCTCATTTAT
Hairpin Sequence:
5'-CCGG-CGCCATGAGCTGCTCATTTAT-CTCGAG-ATAAATGAGCAGCTCATGGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCATGAGCTGCTCATTTATCTCGAGATAAATGAGCAGCTCATGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCATGAGCTGCTCATTTATCTCGAGATAAATGAGCAGCTCATGGCG-3'

Other clones with same target sequence:

(none)