Construct: shRNA TRCN0000024300

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029653.1-2560s1c1
DNA Barcode:
CCCTGTGTACTTCTGTTGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dapk1 (69635)
Gene Description:
death associated protein kinase 1
Transcript:
RefSeq NM_029653.1 (NON-CURRENT)
Match location:
Position 2560 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024300)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTGTACTTCTGTTGCTA
Hairpin Sequence:
5'-CCGG-CCCTGTGTACTTCTGTTGCTA-CTCGAG-TAGCAACAGAAGTACACAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTGTACTTCTGTTGCTACTCGAGTAGCAACAGAAGTACACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTGTACTTCTGTTGCTACTCGAGTAGCAACAGAAGTACACAGGG-3'

Other clones with same target sequence:

  1. TRCN0000322200