Construct: shRNA TRCN0000024346

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144817.1-737s1c1
DNA Barcode:
CCTTTCTATGAAGAAACAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Camk1g (215303)
Gene Description:
calcium/calmodulin-dependent protein kinase I gamma
Transcript:
RefSeq NM_144817.1 (NON-CURRENT)
Match location:
Position 737 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024346)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCTATGAAGAAACAGAA
Hairpin Sequence:
5'-CCGG-CCTTTCTATGAAGAAACAGAA-CTCGAG-TTCTGTTTCTTCATAGAAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCTATGAAGAAACAGAACTCGAGTTCTGTTTCTTCATAGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCTATGAAGAAACAGAACTCGAGTTCTGTTTCTTCATAGAAAGG-3'

Other clones with same target sequence:

(none)