Construct: shRNA TRCN0000024415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_111421.2-184s1c1
DNA Barcode:
GCACTAGATGAAAGGATAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mylk4 (238564)
Gene Description:
myosin light chain kinase family, member 4
Transcript:
RefSeq XM_111421.2 (NON-CURRENT)
Match location:
Position 184 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTAGATGAAAGGATAATT
Hairpin Sequence:
5'-CCGG-GCACTAGATGAAAGGATAATT-CTCGAG-AATTATCCTTTCATCTAGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTAGATGAAAGGATAATTCTCGAGAATTATCCTTTCATCTAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTAGATGAAAGGATAATTCTCGAGAATTATCCTTTCATCTAGTGC-3'

Other clones with same target sequence:

(none)