Construct: shRNA TRCN0000024430

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021461.2-1394s1c1
DNA Barcode:
CCGAGATGCAAACCCATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mknk1 (17346)
Gene Description:
MAP kinase-interacting serine/threonine kinase 1
Transcript:
RefSeq NM_021461.2 (NON-CURRENT)
Match location:
Position 1394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024430)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGATGCAAACCCATGTTT
Hairpin Sequence:
5'-CCGG-CCGAGATGCAAACCCATGTTT-CTCGAG-AAACATGGGTTTGCATCTCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGATGCAAACCCATGTTTCTCGAGAAACATGGGTTTGCATCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGATGCAAACCCATGTTTCTCGAGAAACATGGGTTTGCATCTCGG-3'

Other clones with same target sequence:

  1. TRCN0000321989