Construct: shRNA TRCN0000024517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008847.2-528s1c1
DNA Barcode:
GCCTCTGTCATGCCTGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pip5k1a (18720)
Gene Description:
phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha
Transcript:
RefSeq NM_008847.2 (NON-CURRENT)
Match location:
Position 528 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTGTCATGCCTGTTAAA
Hairpin Sequence:
5'-CCGG-GCCTCTGTCATGCCTGTTAAA-CTCGAG-TTTAACAGGCATGACAGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTGTCATGCCTGTTAAACTCGAGTTTAACAGGCATGACAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTGTCATGCCTGTTAAACTCGAGTTTAACAGGCATGACAGAGGC-3'

Other clones with same target sequence:

(none)