Construct: shRNA TRCN0000024526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_205854.3-1380s1c1
DNA Barcode:
AGACGTTCATTATGGCCGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itpkb (320404)
Gene Description:
inositol 1,4,5-trisphosphate 3-kinase B
Transcript:
RefSeq XM_205854.3 (NON-CURRENT)
Match location:
Position 1380 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACGTTCATTATGGCCGAAT
Hairpin Sequence:
5'-CCGG-AGACGTTCATTATGGCCGAAT-CTCGAG-ATTCGGCCATAATGAACGTCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACGTTCATTATGGCCGAATCTCGAGATTCGGCCATAATGAACGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACGTTCATTATGGCCGAATCTCGAGATTCGGCCATAATGAACGTCT-3'

Other clones with same target sequence:

  1. TRCN0000321746