Construct: shRNA TRCN0000024556

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_284250.2-735s1c1
DNA Barcode:
CCCAAATCTAACCTATGGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Etnk1 (75320)
Gene Description:
ethanolamine kinase 1
Transcript:
RefSeq XM_284250.2 (NON-CURRENT)
Match location:
Position 735 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024556)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAATCTAACCTATGGCTA
Hairpin Sequence:
5'-CCGG-CCCAAATCTAACCTATGGCTA-CTCGAG-TAGCCATAGGTTAGATTTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAATCTAACCTATGGCTACTCGAGTAGCCATAGGTTAGATTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAATCTAACCTATGGCTACTCGAGTAGCCATAGGTTAGATTTGGG-3'

Other clones with same target sequence:

(none)