Construct: shRNA TRCN0000024573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020272.1-2780s1c1
DNA Barcode:
CCACAGTCCTATCCAACGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3cg (30955)
Gene Description:
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
Transcript:
RefSeq NM_020272.1 (NON-CURRENT)
Match location:
Position 2780 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGTCCTATCCAACGAAA
Hairpin Sequence:
5'-CCGG-CCACAGTCCTATCCAACGAAA-CTCGAG-TTTCGTTGGATAGGACTGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGTCCTATCCAACGAAACTCGAGTTTCGTTGGATAGGACTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGTCCTATCCAACGAAACTCGAGTTTCGTTGGATAGGACTGTGG-3'

Other clones with same target sequence:

(none)