Construct: shRNA TRCN0000024575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_193575.2-66s1c1
DNA Barcode:
CGAGATCATCAAGCGATTCGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC268321 (268321)
Gene Description:
similar to nucleoside diphosphate kinase B
Transcript:
RefSeq XM_193575.2 (NON-CURRENT)
Match location:
Position 66 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATCATCAAGCGATTCGA
Hairpin Sequence:
5'-CCGG-CGAGATCATCAAGCGATTCGA-CTCGAG-TCGAATCGCTTGATGATCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATCATCAAGCGATTCGACTCGAGTCGAATCGCTTGATGATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATCATCAAGCGATTCGACTCGAGTCGAATCGCTTGATGATCTCG-3'

Other clones with same target sequence:

(none)